Mouse Genetic Engineering Facility

The Mouse Genetic Engineering Facility (MGEF) provides transgenic mouse services and expertise to help understand gene function during embryonic development and in human disease.

Announcements

Check out our Summer 2017 Newsletter!

Mouse CRISPR News

The MGEF does CRISPR/Cas9 injections to generate mice with precise gene mutations.

The MGEF is part of Sigma-Aldrich's CRISPR Core Partnership Program, which can simplify obtaining microinjection-ready CRISPR reagents for your gene at reduced cost. Contact the MGEF for more information.

Advantages of Mouse Sperm Cryopreservation and IVF

The MGEF performs sperm cryopreservation and IVF. The advantage of sperm cryopreservation over traditional embryo freezing is that only two proven males of reproductive age are required versus 15-20 or more males and females for embryo cryopreservation and it does not require timed matings or multiple freezing sessions. Cryopreservation saves expenses associated with maintaining lines not actively being used and also serves as a backup resource in case of breeding problems, genotyping errors, or disease outbreak.

IVF can be used to obtain mouse lines from international repositories that distribute the lines as cryopreserved sperm. IVF is also a great way to obtain large numbers of mice that are all the same age without the time and expense of having to establish a large transgenic breeder colony.