Mouse Genetic Engineering Facility

The Mouse Genetic Engineering Facility (MGEF) provides services to generate, archive, and distribute transgenic mice.


The MGEF provides a la carte services for CRISPR mouse model design. These include allele design, in vivo testing of gRNA activity, microinjection, founder screening and analysis, off-target analysis, breeding of CRISPR founder mice and F1 analysis, colony management, and sperm and embryo cryopreservation.

The MGEF provides CRISPR injections on a per injection day basis. For simple indel mutation projects, we recommend a single day of injection. For point mutations and tags, we recommend two days of injection. For double-stranded targeting-type vectors, we recommend at least three days of injection.

Cryo Storage. The cryo storage fee has been changed from per investigator to per line. The fee is $53 per line per year.

CRISPR raffle winner – A collaborative project between John Wallingford (Molecular Biosciences) and Ryan Gray (Pediatrics) to study genes involved in cilia function was the winner of the MGEF’s free voucher raffle for the production of a CRISPR knockout mouse.

Calendar Events

Mouse Users Group meeting – February 23rd 2 p.m. ARC 2.204. 

Manu Sebastian D.V.M., Ph.D., from M.D. Anderson Science Park will discuss comparative mouse and human anatomy. He will also provide information on pathology and imaging services available at MD Anderson Science Park. Contact Bill Shawlot ( for more details.

Institute for Cellular and Molecular Biology seminar – March 1st 4 p.m.

The MGEF will host David Zarkower from the University of Minnesota. Dave will speak on Dmrt genes in sex determination and germ cell development.

Transgenic Mouse Technologies Course

The MGEF will hold a half-day short course on Transgenic Mouse Technologies on April 18th from 9 a.m. – 12 p.m. in FNT 1.104 as part of the Center for Computational Biology and Bioinformatics Short Course series. The class will cover practical aspects of generating, maintaining, and analyzing transgenic mice. Registration is free.