The Mouse Genetic Engineering Facility (MGEF) provides transgenic mouse services and expertise to help understand gene function during embryonic development and in human disease.
New method for making loss of function CRISPR mice that makes genotyping quick and easy.
It is relatively easy to generate CRISPR indel mice to make knockout models, however genotyping the resulting mice can be troublesome. Ryan Gray in the Department of Pediatrics at the Dell Medical School at UT Austin has developed a new cassette-based system to alleviate this problem. Working with the MGEF, Ryan has demonstrated the new strategy works efficiently, can be screened rapidly, and the mutation can be passed through the germline. Contact us for more information.
Don’t wait until it is too late.
Don’t lose your valuable transgenic lines due to a natural disaster, pathogen outbreak, or mistakes in genotyping. Make sure your lines are backed up. The MGEF recommends cryopreserving sperm for efficient backup. Sperm cryopreservation requires only two male mice that are ideally between 3-6 months of age and are proven breeders.
Deficiency in the manganese efflux transporter Slc30a10 induces severe hypothyroidism in mice. Hutchens S, Liu C, Jursa T, Shawlot W, Chaffee BK, Yin W, Gore AC, Aschner M, Smith DR, Mukhopadhyay S. J. Biol Chem., 292:9760-9773, 2017.
Hypothyroidism induced by loss of the manganese efflux transporter Slc30a10 may be explained by reduced thyroxine production. Liu C, Hutchens S, Jursa T, Shawlot W, Polishchuk EV, Polishchuk RS, Dray BK, Gore AC, Aschner M, Smith DR, Mukhopadhyay S. J. Biol Chem., in press.