Electron Microscopy

SEM

Zeiss Supra 40V Scanning Electron Microscope (SEM)

Based on Zeiss' GEMINI field emission SEM column, the Zeiss Supra 40V Scanning Electron Microscope (SEM) provides ultra high resolution at low kV. Additionally, the variable presser chamber allows for imaging of non-conductive samples. The large chamber and user friendly software make this a versatile easy to use instrument. Data retrieval on CD or USB.

Cryo-SEM is also available, but requires special training and instrument prep.

For inquiries about SEM or cryo-SEM, please contact Dwight Romanovic.

Instrument Access: Assisted use and training for individual use are available.  To schedule assisted use or training, please fill out the Access Request Form and email it to Dwight Romanovic.

Fees: Trained user: $52/hour. Assistated user: $92/hour.

SEM PREP

Instruments available to assist with TEM Prep

Tousimis Samdri 790 CPD available for critical point drying.  Critical point drying is still the standard by which other structure preservation techniques are measured. Sample holders for bulk or large specimens, TEM grids, or coverslips.

Cressington 208 Benchtop Sputter Coater can provide ultra-thin (nm scale) grain free coatings for high resolution SEM applications. The attached thickness monitor has a resolution on 0.1 nm, for reproducible coatings. We keep a stock of metal targets including Au/Pd, Pt/Pd, Cr, and Ir.

Bal-Tec MED 20 High Vacuum Coater is a quick turbo-pumped unit with accessory for rotary and directional electron beam evaporation. Useful for low-angle high-resolution tungsten shadowing.

Instrument Access: Training for individual use is available.  To schedule training, please fill out the Access Request Form and email it to Dwight Romanovic.

Fees: Critical Point Dryer: $15/run

Sputter coater: $2.50/run + the costs of metal (please inquire regarding metal fees)

High Vacuum Coater: $2.50/run

Users are required to purchase their own consumables.

TEM

FEI Tecnai Transmission Electron Microscope (TEM)

The FEI Tecnai Transmission Electron Microscope (TEM) is a 20-120kV instrument that we typically operate at 80kV for improved contrast of biological specimens.  The BioTwin lens configuration enhances specimen contrast at the medium magnification used with resin-embedded tissue while permitting sub-nanometer resolution with the right specimen and instrument configuration. The Tecnai is fitted with a top mount AMT Advantage HR 1kX1k digital camera which is sensitive at low illumination and has FFT assistance for easier focusing and astigmatism compensation. The microscope is reliable and easy to use. With its convenient embedded automation, the Tecnai Spirit TEM automatically performs many routine operating procedures. It can automatically align and tune the instrument and offers automated image capture for e-tomography and photomontage.  Images can be retrieved on CD, 250Mb PC Zips, or USB.

Cryo-TEM is available, but requires special training and instrument prep. 

For inquiries about the TEM or cryo-TEM, please contact Dwight Romanovic.

Instrument Access: Assisted use and training for individual use are available.  To schedule assisted use or training, please fill out the Access Request Form and email it to Dwight Romanovic.

Fees: Trained user: $65/hour. Assisted use: $105/hour

TEM PREP

Instruments available to assist with TEM Prep

Pelco BioWave (Microwave Prep)

Leica EM AFS and Leica EM CPC Freeze Substitution System

Available cryo-preparation for TEM (or SEM) by immersion (here typically propane), metal mirror, and other methods, and automated freeze substitution and embedding (here often HM20 and UV low temperature polymerization), especially for enhanced gold-bead ICC work

Emitech Glow Discharge

Bal-Tec MED 20 High Vacuum Coater

This quick turbo-pumped unit with accessory for rotary and directional electron beam evaporation. Useful for low-angle high-resolution tungsten shadowing.

Instrument Access: Training for individual is available.  To schedule training, please fill out the Access Request Form and email it to Dwight Romanovic.

Fees: High Vacuum Coater: $2.50/run

Microwave prep station, Glow Discharge, & freeze substitution unit do not have a user fee, but do require training.

Users are required to purchase their own consumables.